Cynthia K. Holland

Cynthia Holland

Assistant Professor of Biology

413-597-2746
Hopper Science Center Rm 132
At Williams since January of 2020

Education

Ph.D. Washington University, Biology

Areas of Expertise

Protein Biochemistry & Plant Metabolism

Scholarship/Creative Work

About half of all modern pharmaceuticals are derived from or inspired by compounds found in nature, and many of these molecules are still extracted from plants. Plants typically produce these chemicals as toxins to deter herbivores, like insects and animals, but the enzymes that they use to produce these natural products remain largely unknown. The Holland lab uses molecular biology and protein biochemistry techniques to identify and characterize the biosynthetic pathways of commercially and pharmaceutically relevant plant metabolites.

NSF-funded research on anthranilate metabolism

To defend themselves against herbivores, plants such as grape, maize, citrus, and strawberry emit a volatile called methyl anthranilate, which attracts parasitic insects and acts as a natural bird deterrent. While at least ten plant families produce methyl anthranilate, all plants synthesize the precursor, anthranilate, as an intermediate in the biosynthesis of the amino acid tryptophan (Trp), which is essential for plant growth and development. Some plants siphon anthranilate away from the Trp pathway by methylating it, forming the volatile O-methyl anthranilate, while the Citrus family generates N-methyl anthranilate as a precursor to toxic acridone alkaloids. The goal of this research is to understand how plants regulate anthranilate metabolism at the interface between amino acid synthesis and defense metabolism. Since methyl anthranilate is used commercially as a grape flavoring agent in the food and beverage industry and as an anti-avian spray for fields and crops, knowledge gained from these experiments may have implications in biotechnology and agriculture.

Cardenolide biosynthesis in wallflower

Cardenolides are a chemically diverse group of natural products that act as allosteric inhibitors of Na+,K+-ATPase, an essential membrane ion transporter that is found in almost all animal cells. The pharmaceutical cardenolide digoxin is used to treat heart arrhythmias and is on the World Health Organization’s list of essential medicines, but the cardenolide biosynthetic pathway has yet to be investigated. Using a recently published genome for a cardenolide-producing species of wallflower (Erysimum cheiranthoides), our current efforts are focused on identifying and characterizing candidate genes using genomics, protein biochemistry, and analytical chemistry techniques.

Current Lab Members:

  • Matias Enriquez ’23
  • Miriam Li ’23
  • Hisham Tadfie ’23
  • Michael Fallon ’24
  • John Lucey ’24

Former Thesis Students:

  • Rebecca Brody ’22, pursuing an M.Phil. at Cambridge
  • Madeline Dyke ‘21.5, Co-founder + Operations Director of Climate Changemakers
  • Jessica Hem ’21, Research Technician in the Mullally Lab at Brigham and Women’s Hospital

Selected publications:

Holland CK and Hisham Tadfie ’23 A structure-guided computational screening approach for predicting plant enzyme-metabolite interactions. In Methods in Enzymology, in press.

Lopez-Nieves S, El-Azaz J, Men Y, Holland CK, Feng T, Brockington S, Jez JM, Maeda H. Two independently evolved natural mutations additively deregulate TyrA enzymes and boost tyrosine production in planta. Plant J. 109(4): 844-55.

Züst T, Strickler SR, Powell AF, Mabry ME, An H, Mirzaei M, York T, Holland CK, Kumar P, Erb M, Petschenka G, Gómez J-M, Perfectti F, Müller C, Pires JC, Mueller LA, Jander G (2020) Independent evolution of ancestral and novel defenses in a genus of toxic plants (Erysimum, Brassicaceae). eLife 9:e51712

Schenck CA, Holland CKSchneider MR, Men Y, Lee SG, Jez JM, Maeda HA (2017) Molecular basis of the evolution of alternative tyrosine biosynthesis pathways in plants.  Nature Chem Biol 13, 1029-35.

Current Committees

  • British Post Graduate Advisory Committee